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Protein production in E. coli

With tailored services and expert guidance, our team will help you define the most relevant strategy for an efficient expression of your protein in the Escherichia coli expression system.

GTP Bioways CDMO - Formulation Development

Matchless expertise in E. coli protein expression

GTP Bioways has matchless expertise in Escherichia coli protein expression, gained over 20 years. Our experienced team has successfully produced hundreds of challenging proteins using cytoplasmic, periplasmic and soluble or insoluble (inclusion bodies) expression strategies in Escherichia coli.

Whether your project aims to develop a therapeutic or vaccine protein, obtain materials for research studies or come up with a diagnostic tool, we will be able to provide a customised approach and build efficient processes for the successful production of your target protein in Escherichia coli.

We will take care of every detail to ensure the success of your protein production challenge

GTP Bioways provides customised expression services to each protein project. Our expert scientific team will design a specific approach based on your development stage, whether you need research material to complete your POC studies or are looking for GMP manufacturing capacities to bring your project to the clinic (small scale research batch, large scale non-GMP material or cGMP batch).

Unique tools for optimised E. coli protein expression

Drawing on our experience expressing a wide range of proteins (enzymes, antigens, cytokines fusion proteins, antibody fragments, etc…), our experts have developed advanced tools for enhanced expression of proteins using E. coli. We have developed our own gene optimisation programme that takes into account parameters that we consider critical for improved protein expression in E. coli and that are usually left aside by gene synthesis companies.

We have also developed our own IP-free vectors with various features designed to improve protein expression and stability.

A complete development and manufacturing solution for your biopharmaceuticals
Microbial CDMO activities

Unique tools for optimised E. coli protein expression

We have the expertise and capabilities to manage complete E. coli production projects from gene synthesis through to milligram to decagram-scale batch production .

Our custom services include:

GTP Bioways has expertise with other expression systems

We excel at protein production with all prominent industrial microbial, mammalian and insect cell expression systems such as P. pastoris, CHO, HEK and Sf9.

Yeast

Our team has a strong experience with various recognised P. pastoris expression systems.

Mammalian cells

We offer both transient and stable expression of proteins and antibodies in CHO and HEK cells.

Insect cells

We are experts in the production of target, reagent and vaccine proteins using insect cells.