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Protein production in E. coli

Our team offers expert guidance and tailored services to define the most relevant strategy for an efficient expression of your protein in Escherichia coli.
Commercial manufacturing of small batches for nanodrugs

Unique expertise in E. coli protein expression

GTP Bioways has a matchless expertise in Escherichia coli protein expression. Our experienced team has successfully produced hundreds of challenging proteins and developed efficient processes for cytoplasmic, periplasmic and soluble or insoluble expression of proteins in Escherichia coli.

Whether your project aims to develop a therapeutic or vaccine protein, to obtain materials for research studies or to develop a diagnostic tool, we will be able to propose a customised approach for the successful production of your protein in Escherichia coli.

Integrated services for protein production in Escherichia coli

We have the expertise and capabilities to manage complete E. coli production projects from gene synthesis through to milligram to decagram-scale batch production.

Our custom services include:

    • E. coli strain development, including expression vector design & construction
    • Yield optimisation in small-scale feasibility studies for soluble/insoluble (inclusion bodies), periplasmic/cytoplasmic expressions
    • Screening and optimisation of critical parameters for solubilisation and refolding.
    • Development of batch and fed-batch fermentations
    • Development/optimisation of the purification process
GTP Biologics

Years

Our expertise: a guarantee of success for your programme

With expertise gained over 20 years, our team offers expert guidance to define a relevant strategy for an efficient expression of your protein in E. coli. We have successfully produced hundreds of challenging proteins and developed efficient processes for cytoplasmic, periplasmic and soluble or insoluble expression of proteins in E. coli.
Advanced Biodesign

Unique tools for optimised E. coli protein expression

Our experts have developed advanced tools for enhanced expression of your protein in E. coli.

When gene synthesis is required, we use our own gene optimisation programme. This tool takes into account parameters that we consider critical for improved protein expression in E. coli that are usually left aside by gene synthesis companies.

We also offer IP-free vectors with different features, all designed to improve protein expression and stability.

GTP Bioways has expertise with other expression systems

We excel at protein production with all prominent industrial microbial, mammalian and insect cell expression systems such as P. pastoris, CHO, HEK and Sf9.

Yeast

Our team has a strong experience with various recognised P. pastoris expression systems.

Mammalian cells

We offer both transient and stable expression of proteins and antibodies in CHO and HEK cells.

Insect cells

We are experts in the production of target, reagent and vaccine proteins using insect cells.