Protein production in E. coli
Integrated services for protein production in Escherichia coli
We have the expertise and capabilities to manage complete E. coli production projects from gene synthesis through to milligram to decagram-scale batch production.
Our custom services include:
- E. coli strain development, including expression vector design & construction
- Yield optimisation in small-scale feasibility studies for soluble/insoluble (inclusion bodies), periplasmic/cytoplasmic expressions
- Screening and optimisation of critical parameters for solubilisation and refolding.
- Development of batch and fed-batch fermentations
- Development/optimisation of the purification process
Our expertise: a guarantee of success for your programme
Unique tools for optimised E. coli protein expression
Our experts have developed advanced tools for enhanced expression of your protein in E. coli.
When gene synthesis is required, we use our own gene optimisation programme. This tool takes into account parameters that we consider critical for improved protein expression in E. coli that are usually left aside by gene synthesis companies.
We also offer IP-free vectors with different features, all designed to improve protein expression and stability.
GTP Bioways has expertise with other expression systems
We excel at protein production with all prominent industrial microbial, mammalian and insect cell expression systems such as P. pastoris, CHO, HEK and Sf9.
Our team has a strong experience with various recognised P. pastoris expression systems.
We offer both transient and stable expression of proteins and antibodies in CHO and HEK cells.
We are experts in the production of target, reagent and vaccine proteins using insect cells.