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Microbial Strain Development

GTP Bioways has extensive experience developing microbial strains for the GMP manufacturing of biologics.

Commercial manufacturing of small batches for nanodrugs

GMP-compliant microbial strain development services

We know how important it is for the successful development of your biotherapeutic candidate to obtain an efficient production strain. Drawing on many years of experience, we offer flexible and robust microbial strain development services. Our team will handle the entire development from synthesis to the generation of a stable and thoroughly characterised Research Cell Bank (RCB).

With a complete history of all materials (microbial strain, plasmid) and full traceability of every operation performed, you will have all the documentation required by the regulatory authorities.

Extensive knowledge in E.coli strain development

We offer optimised IP-free vectors designed for improved protein expression and stability and have successfully developed processes for a range of expression strategies: cytoplasmic, periplasmic, soluble and insoluble (inclusion bodies).

Advanced Biodesign

Expert E. coli strain development services

To develop high-producing E. coli strains, our approach is to screen a complete set of parameters, from which we select the strain and expression conditions allowing for the highest productivity and the production of a protein with the required quality attributes.

Depending on the specificities of your molecule, we will design a custom package including the screening of different parameters such as gene sequences, promoters, expression strains, expression strategies (soluble/insoluble, periplasmic/cytoplasmic, co-expression with chaperones), plasmid stabilisation sequences and expression conditions (induction, media, temperature and kinetic).

P. pastoris strain development services

P. pastoris (renamed Komagataella phaffii) is an established, FDA-approved and highly competitive expression host.

Our team has successfully produced a diversity of recombinant proteins (enzymes, cytokines, antibody-derived fragments…) using the methanol-inducible AOX promoter or the constitutive GAP promoter.

By working on the design of an optimised strain for the production of your specific protein and performing an extended screening of up to several thousand clones, we will be able to identify the highest producers. We will use the adaquate analytical methods all along the strain development process, ensuring we select a clone that will produce a molecule with the expected quality attributes.

GTP Biologics

Integrated CDMO services from R&D to cGMP manufacturing

Our microbial strain development services can be used as stand-alone services, but are more often integrated into complete process development & GMP manufacturing programmes.

Advanced Biodesign

Discover more about GTP Bioways services

Microbial Manufacturing

Process Development

Microbial Manufacturing

GMP Manufacturing

Biologics

Analytics & Formulation