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Production in Pichia pastoris

Our team offers expert guidance and tailored services to define the most relevant strategy for an efficient expression of your protein in P. pastoris.
GTP Bioways CDMO - Antibody drug conjugate development

Expertise with a range of P. pastoris expression systems

Pichia pastoris (now Komagataella phaffii) is a highly competitive host for the production of recombinant proteins.

This FDA-approved system shows a high secretion capacity of heterologous proteins and can be grown to high cell densities, making it one of the most cost-efficient systems for protein production.

Our team has successfully produced a diversity of proteins (enzymes, antibody fragments, antigens…) in different P. pastoris systems, with inducible or constitutive expression.

A passionate team dedicated to the success of your programme

Customised services for efficient P. pastoris protein expression

GTP Bioways offers customised services that span the entire range from gene or sequence to highly productive expression strains and processes:

    • Generation of P. pastoris strain, including expression vector design & construction and when relevant, screening of strains, promoters and signal sequences
    • Screening of up to several thousand clones to identify high producers
    • Development of fed-batch fermentations
    • Development/optimisation of the purification process
Cell line Development process

GTP Bioways has expertise with other expression systems

We excel at protein production with all prominent industrial microbial, mammalian and insect cell expression systems such as P. pastoris, CHO, HEK and Sf9.

E. coli

We have successfully developed protein expression processes in E. coli with a diversity of strategies.

Mammalian cells

We offer both transient and stable expression of proteins and antibodies in CHO and HEK cells.

Insect cells

We are experts in the production of target, reagent and vaccine proteins using insect cells.