Microbial Strain Development
GTP Bioways has extensive experience developing microbial strains for the GMP manufacturing of biologics.
Expert E. coli strain development services
To develop high-producing E. coli strains, our approach is to screen a complete set of parameters, from which we select the strain and expression conditions allowing for the highest productivity and the production of a protein with the required quality attributes.
Depending on the specificities of your molecule, we will design a custom package including the screening of different parameters such as gene sequences, promoters, expression strains, expression strategies (soluble/insoluble, periplasmic/cytoplasmic, co-expression with chaperones), plasmid stabilisation sequences and expression conditions (induction, media, temperature and kinetic).
P. pastoris strain development services
P. pastoris (renamed Komagataella phaffii) is an established, FDA-approved and highly competitive expression host.
Our team has successfully produced a diversity of recombinant proteins (enzymes, cytokines, antibody-derived fragments…) using the methanol-inducible AOX promoter or the constitutive GAP promoter.
By working on the design of an optimised strain for the production of your specific protein and performing an extended screening of up to several thousand clones, we will be able to identify the highest producers. We will use the adaquate analytical methods all along the strain development process, ensuring we select a clone that will produce a molecule with the expected quality attributes.
Integrated CDMO services from R&D to cGMP manufacturing
Our microbial strain development services can be used as stand-alone services, but are more often integrated into complete process development & GMP manufacturing programmes.